gel red dye (Biotium)
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gel red dye
Gel Red Dye, supplied by Biotium, used in various techniques. Bioz Stars score: 97/100, based on 3809 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gel+red+nucleic+acid+gel+stain/GelRed+Nucleic+Acid+Gel+Stain/pm42107190-83-100-103
Average 97 stars, based on 3809 article reviews
Gel Red Dye, supplied by Biotium, used in various techniques. Bioz Stars score: 97/100, based on 3809 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gel+red+nucleic+acid+gel+stain/GelRed+Nucleic+Acid+Gel+Stain/pm42107190-83-100-103
Average 97 stars, based on 3809 article reviews
gel red dye - by Bioz Stars,
2026-10
97/100 stars
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Polymerase Chain Reaction:Article Title: Highly efficient production of transgenic rats with long DNA insertions using piggyBac transposase mRNA and piezo-assisted microinjection Article Snippet: The reaction parameters were as follows: tdTomato , one cycle at 94°C for 1 min and 35 cycles at 98°C for 10 s, 58.7°C for 15 s, and 68°C for 30 s using Tks Gflex DNA Polymerase; Egfp , one cycle at 94°C for 1 min and 35 cycles at 98°C for 10 s, 68°C for 30 s using Tks Gflex DNA Polymerase; 5’ITR-Thy1 promoter and rGB pA-3’ITR , one cycle at 95°C for 10 min and 35 cycles at 98°C for 30 s, 55°C for 1 min, and 72°C for 30 s using Bio TAQ (BIO-21040, NIPPON Genetics, Tokyo, Japan). .. The PCR products were electrophoresed on 2% agarose gels with TBE and visualized using Midori Green Advanced (1:10,000; NE-MG04; NIPPON Genetics) or Article Title: Identification and Characterization of Novel PRNP Haplotypes in the Assaf Sheep Breed from Palestine Article Snippet: Scrapie is a fatal neurodegenerative disease affecting sheep and goats, strongly influenced by genetic variations in the prion protein gene (PRNP).. This study aimed to identify and characterize PRNP polymorphism in the Assaf sheep breed from Palestine, an important dairy breed not previously studied for scrapie-related genetic diversity.. Genomic DNA was extracted from 257 Assaf sheep, and the PRNP coding region (specifically exon 3 encompassing the open reading frame with focus on codons 136 (A/V), 154 (R/H), and 171 (R/Q/H/K) was sequenced to identify polymorphisms linked to scrapie susceptibility. Article Title: Highly efficient production of transgenic rats with long DNA insertions using piggyBac transposase mRNA and piezo-assisted microinjection. Article Snippet: The reaction parameters were as follows: tdTomato, one cycle at 94°C for 1 min and 35 cycles at 98°C for 10 s, 58.7°C for 15 s, and 68°C for 30 s using Tks Gflex DNA Polymerase; Egfp, one cycle at 94°C for 1 min and 35 cycles at 98°C for 10 s, 68°C for 30 s using Tks Gflex DNA Polymerase; 5’ITR-Thy1 promoter and rGB pA-3’ITR, one cycle at 95°C for 10 min and 35 cycles at 98°C for 30 s, 55°C for 1 min, and 72°C for 30 s using Bio TAQ (BIO-21040, NIPPON Genetics, Tokyo, Japan). .. The PCR products were electrophoresed on 2% agarose gels with TBE and visualized using Midori Green Advanced (1:10,000; NE-MG04; NIPPON Genetics) or Staining:Article Title: Highly efficient production of transgenic rats with long DNA insertions using piggyBac transposase mRNA and piezo-assisted microinjection Article Snippet: The reaction parameters were as follows: tdTomato , one cycle at 94°C for 1 min and 35 cycles at 98°C for 10 s, 58.7°C for 15 s, and 68°C for 30 s using Tks Gflex DNA Polymerase; Egfp , one cycle at 94°C for 1 min and 35 cycles at 98°C for 10 s, 68°C for 30 s using Tks Gflex DNA Polymerase; 5’ITR-Thy1 promoter and rGB pA-3’ITR , one cycle at 95°C for 10 min and 35 cycles at 98°C for 30 s, 55°C for 1 min, and 72°C for 30 s using Bio TAQ (BIO-21040, NIPPON Genetics, Tokyo, Japan). .. The PCR products were electrophoresed on 2% agarose gels with TBE and visualized using Midori Green Advanced (1:10,000; NE-MG04; NIPPON Genetics) or Article Title: Seroprevalence, Associated Factors, and Molecular Detection of Toxoplasma gondii Infection in Brazilian Gold Miners Working Informally in French Guiana Article Snippet: .. The nPCR products were visualized on a 1% agarose gel, stained with a solution of Article Title: Identification and Characterization of Novel PRNP Haplotypes in the Assaf Sheep Breed from Palestine Article Snippet: Scrapie is a fatal neurodegenerative disease affecting sheep and goats, strongly influenced by genetic variations in the prion protein gene (PRNP).. This study aimed to identify and characterize PRNP polymorphism in the Assaf sheep breed from Palestine, an important dairy breed not previously studied for scrapie-related genetic diversity.. Genomic DNA was extracted from 257 Assaf sheep, and the PRNP coding region (specifically exon 3 encompassing the open reading frame with focus on codons 136 (A/V), 154 (R/H), and 171 (R/Q/H/K) was sequenced to identify polymorphisms linked to scrapie susceptibility. Article Title: Immune priming reshapes the microbiota and modulates pathogen dynamics in the Manila clam ( Ruditapes philippinarum ) Article Snippet: Thermal cycling conditions consisted of an initial denaturation at 95 °C for 3 min, followed by 20 amplification cycles at 98 °C for 20 s, 57 °C for 30 s, and 72 °C for 75 s, with a final extension at 72 °C for 5 min, using 2 × KAPA HiFi HotStart Ready Mix polymerase (Roche, Switzerland). .. The expected amplicon size (~1,500 bp) was verified by electrophoresis on a 1% (w/v) agarose gel (Lonza, Switzerland) stained with Article Title: Gut microbiota diversity among humans, elephants, livestock and wild herbivores in Chitwan National Park bears implications for conservation medicine. Article Snippet: .. Aliquots of 1.5 μl each were run on a 1.5% (w/v) agarose gel stained with Article Title: Comparing the respiratory tract microbiome in captive elephants and humans in Chitwan National Park: Implications for conservation medicine. Article Snippet: We carried out triplicate PCR reactions in a volume of 2.5 μl [36] using 1.25 μl of 2X Kapa HiFi Hotstart Ready Mix (Roche, Wilmington, MA, USA) 0.5 μl of each 1 pmol illumina forward (16s_F) and (16s_R) reverse primers and 0.25 μl of template DNA. .. The thermal profile was: 3 min at 95◦C, 35 cycles at 98◦C for 30 s, 65◦C for 30 s and 72◦C for 20 s, and final extension at 72◦C for 3 min. A 1.5 μl aliquot of the 7.5 μl pooled volume was run on 1.5 % (w/v) agarose gel stained with Article Title: Highly efficient production of transgenic rats with long DNA insertions using piggyBac transposase mRNA and piezo-assisted microinjection. Article Snippet: The reaction parameters were as follows: tdTomato, one cycle at 94°C for 1 min and 35 cycles at 98°C for 10 s, 58.7°C for 15 s, and 68°C for 30 s using Tks Gflex DNA Polymerase; Egfp, one cycle at 94°C for 1 min and 35 cycles at 98°C for 10 s, 68°C for 30 s using Tks Gflex DNA Polymerase; 5’ITR-Thy1 promoter and rGB pA-3’ITR, one cycle at 95°C for 10 min and 35 cycles at 98°C for 30 s, 55°C for 1 min, and 72°C for 30 s using Bio TAQ (BIO-21040, NIPPON Genetics, Tokyo, Japan). .. The PCR products were electrophoresed on 2% agarose gels with TBE and visualized using Midori Green Advanced (1:10,000; NE-MG04; NIPPON Genetics) or Article Title: Characterization of antibiotic resistance of isolates of Klebsiella pneumoniae causing health care-associated infections (HAIs) in hospitals in the Department of Nariño. Article Snippet: .. The gel was treated with Agarose Gel Electrophoresis:Article Title: Seroprevalence, Associated Factors, and Molecular Detection of Toxoplasma gondii Infection in Brazilian Gold Miners Working Informally in French Guiana Article Snippet: .. The nPCR products were visualized on a 1% agarose gel, stained with a solution of Article Title: Immune priming reshapes the microbiota and modulates pathogen dynamics in the Manila clam ( Ruditapes philippinarum ) Article Snippet: Thermal cycling conditions consisted of an initial denaturation at 95 °C for 3 min, followed by 20 amplification cycles at 98 °C for 20 s, 57 °C for 30 s, and 72 °C for 75 s, with a final extension at 72 °C for 5 min, using 2 × KAPA HiFi HotStart Ready Mix polymerase (Roche, Switzerland). .. The expected amplicon size (~1,500 bp) was verified by electrophoresis on a 1% (w/v) agarose gel (Lonza, Switzerland) stained with Article Title: Gut microbiota diversity among humans, elephants, livestock and wild herbivores in Chitwan National Park bears implications for conservation medicine. Article Snippet: .. Aliquots of 1.5 μl each were run on a 1.5% (w/v) agarose gel stained with Article Title: Comparing the respiratory tract microbiome in captive elephants and humans in Chitwan National Park: Implications for conservation medicine. Article Snippet: We carried out triplicate PCR reactions in a volume of 2.5 μl [36] using 1.25 μl of 2X Kapa HiFi Hotstart Ready Mix (Roche, Wilmington, MA, USA) 0.5 μl of each 1 pmol illumina forward (16s_F) and (16s_R) reverse primers and 0.25 μl of template DNA. .. The thermal profile was: 3 min at 95◦C, 35 cycles at 98◦C for 30 s, 65◦C for 30 s and 72◦C for 20 s, and final extension at 72◦C for 3 min. A 1.5 μl aliquot of the 7.5 μl pooled volume was run on 1.5 % (w/v) agarose gel stained with Amplification:Article Title: Immune priming reshapes the microbiota and modulates pathogen dynamics in the Manila clam ( Ruditapes philippinarum ) Article Snippet: Thermal cycling conditions consisted of an initial denaturation at 95 °C for 3 min, followed by 20 amplification cycles at 98 °C for 20 s, 57 °C for 30 s, and 72 °C for 75 s, with a final extension at 72 °C for 5 min, using 2 × KAPA HiFi HotStart Ready Mix polymerase (Roche, Switzerland). .. The expected amplicon size (~1,500 bp) was verified by electrophoresis on a 1% (w/v) agarose gel (Lonza, Switzerland) stained with Electrophoresis:Article Title: Immune priming reshapes the microbiota and modulates pathogen dynamics in the Manila clam ( Ruditapes philippinarum ) Article Snippet: Thermal cycling conditions consisted of an initial denaturation at 95 °C for 3 min, followed by 20 amplification cycles at 98 °C for 20 s, 57 °C for 30 s, and 72 °C for 75 s, with a final extension at 72 °C for 5 min, using 2 × KAPA HiFi HotStart Ready Mix polymerase (Roche, Switzerland). .. The expected amplicon size (~1,500 bp) was verified by electrophoresis on a 1% (w/v) agarose gel (Lonza, Switzerland) stained with Imaging:Article Title: Gut microbiota diversity among humans, elephants, livestock and wild herbivores in Chitwan National Park bears implications for conservation medicine. Article Snippet: .. Aliquots of 1.5 μl each were run on a 1.5% (w/v) agarose gel stained with Article Title: Comparing the respiratory tract microbiome in captive elephants and humans in Chitwan National Park: Implications for conservation medicine. Article Snippet: We carried out triplicate PCR reactions in a volume of 2.5 μl [36] using 1.25 μl of 2X Kapa HiFi Hotstart Ready Mix (Roche, Wilmington, MA, USA) 0.5 μl of each 1 pmol illumina forward (16s_F) and (16s_R) reverse primers and 0.25 μl of template DNA. .. The thermal profile was: 3 min at 95◦C, 35 cycles at 98◦C for 30 s, 65◦C for 30 s and 72◦C for 20 s, and final extension at 72◦C for 3 min. A 1.5 μl aliquot of the 7.5 μl pooled volume was run on 1.5 % (w/v) agarose gel stained with |